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KMID : 0620919990310040174
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Experimental & Molecular Medicine
1999 Volume.31 No. 4 p.174 ~ p.178
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Tanshinone ⅡA, an ingredient of Salvia miltiorrhiza BUNGE, induces apoptosis in human leukemia cell lines through the activation of caspase-3
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Hyun Jea Sung/Hyun Jea Sung
Sun Mi Choi/Yoo Sik Yoon/Kyu Suk An
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Abstract
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Tanshinone II-A is a derivative of phenanthrene-quinone isolated from Salvia miltiorrhiza BUNGE, a traditional herbal medicine that is known to induce antiinflammatory, anti-oxidative and cytotoxic activity. We have examined cellular effects of Tanshione II-A on HL60 human promyelocytic leukemic cells and K562 human erythroleukemic cells. Tanshione II-A induced a dose- and time-dependent DNA fragmentation into the multiples of 180 bp and specific proteolytic cleavage of poly(ADP-ribose) polymerase in both cell lines. PI-staining and flow cytometry analysis of K562 cells following Tanshione II-A treatment showed an increase of the cells possessing hypodiploid DNA indicative of apoptotic state of cells. Caspase-3 activity was significantly increased during Tanshinone II-A treatment of both HL60 and K562 cells, whereas caspase-1 activity was not changed. These results suggest that Tanshione II-A induced HL60 and K562 cellular apoptosis that may be associated with the selective members of caspase family.
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KEYWORD
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apoptosis, caspase-3, leukemia, Salvia miltiorrhiza BUNGE, tanshinone Ⅱ-A,
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